Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide

Catalog #MHC-HE460B

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$341.00

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Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HE460B) Summary

Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide.
Product Background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of -the MHC Class I and YLEPGPVTA peptide of -the gp100.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein	HLA-A*02:01&B2M&GP100 (YLEPGPVTA)
Synonyms	HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide
Molecular Weight	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human HLA-A02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
	Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC.

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