Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer Protein (MHC-HE008B)
Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer Protein (MHC-HE008B)
Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer Protein (MHC-HE008B)
  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLVVVGAGGV peptide
Catalog #MHC-HE008B
Regular price $563.00
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Product Description
Recombinant Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLVVVGAGGV peptide.
Product Background
Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target.
Product Category
Recombinant Protein
Target
HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer
Synonyms
MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form
Monomer
Allele
HLA-A*02:01
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLVVVGAGGV peptide
Molecular Weight
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 33.2ng/ml determined by ELISA.
Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&KRAS WT (KLVVVGAGGV) Monomer is greater than 90% as determined by SEC-HPLC.

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