Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein (MHC-HM460B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KMVELVHFL peptide

Catalog: MHC-HM460B

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Product Description Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KMVELVHFL peptide.

Product Background

Melanoma antigen gene (MAGE)-A2 (MAGEA2) is a member of the MAGE-A family proteins widely studied for cancer vaccine development and identification of tumor markers. MAGEA2 plays an oncogenic role in glioma progression, and they provide insight into MAGEA2 application as a novel predictor of clinical outcomes and a potential glioma biomarker.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer
Accession
MHC Form Monomer
Allele HLA-A*02:01

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KMVELVHFL peptide
Molecular Weight The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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