Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer Protein (MHC-HM461B)
Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer Protein (MHC-HM461B)
Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer Protein (MHC-HM461B)
Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer Protein (MHC-HM461B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVAELVHFL peptide
Catalog #MHC-HM461B
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Product Description
 Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide.
Product Background
 Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.
Product Category
 Recombinant Protein
Target
 HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer
Synonyms
 MAGE-A3
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVAELVHFL peptide
Molecular Weight
 The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.

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