• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide
Catalog #MHC-HE034B
Price
Regular price $34100
Regular price Sale price $34100
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein (MHC-HE034B) Summary

Product Description

 Recombinant Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide.

Product Background

 Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.

HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Specifications

Protein
 HLA-A*02:01&B2M&MART-1 (EAAGIGILTV)
Synonyms
 MART1; LB39-AA; SK29-AA; Melan-A; MLANA;LB39AA; SK29AA
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide
Molecular Weight
 The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA. Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
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