Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)
Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)
Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)
Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
  • Activity: Immobilized HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide
Catalog #MHC-HM435B
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Product Description
 Recombinant Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and ELAGIGILTV peptide.
Product Background
 Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
Product Category
 Recombinant Protein
Target
 HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer
Synonyms
 MART1; LB39-AA; SK29-AA; Melan-A; MLANA
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide
Molecular Weight
 The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 >95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test).
Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.

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