Name: Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)
Brand: KactusBio
SKU: MHC-HM435B-100UG
Price: 310.0 USD
Availability: InStock

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)

Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: >95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide

Catalog #MHC-HM435B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and ELAGIGILTV peptide.
Product Background	Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer
Synonyms	MART1; LB39-AA; SK29-AA; Melan-A; MLANA
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&ELAGIGILTV
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide
Molecular Weight	The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	>95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.