Name: Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)
Brand: KactusBio
SKU: MHC-HM435B-100UG
Price: 341.0 USD
Availability: InStock

SDS-PAGE gel image showing a purified protein band at the expected molecular weight alongside a protein ladder for size reference.

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B)

Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA.

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide

Catalog #MHC-HM435B

Size

100UG

500UG

Order by 4pm ET for delivery on:

[{"id":45858630795486,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"MHC-HM435B-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human HLA-A*02:01\u0026B2M\u0026MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B) - 100UG","public_title":"100UG","options":["100UG"],"price":34100,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":45858633253086,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"MHC-HM435B-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human HLA-A*02:01\u0026B2M\u0026MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B) - 500UG","public_title":"500UG","options":["500UG"],"price":137500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435B) Summary

Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and ELAGIGILTV peptide.
Product Background	Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Specifications

Protein	HLA-A*02:01&B2M&MART-1 (ELAGIGILTV)
Synonyms	MART1; LB39-AA; SK29-AA; Melan-A; MLANA
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&ELAGIGILTV
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide
Molecular Weight	The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA.
	Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.