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Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein (MHC-HE446B) Summary
Product Description |
Recombinant Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide. |
Product Background |
NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Specifications
Protein |
HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) |
Synonyms |
MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Avi Tag with the EC50 of 20.6ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 0.2M NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Avi Tag with the EC50 of 20.6ng/ml determined by ELISA. |
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Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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