Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein (MHC-HE027B)
  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide
Catalog #MHC-HE027B
Regular price $341.00
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Product Description

Recombinant Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide.

Product Background

A key regulator of cell division is polo-like kinase 1 (PLK1), a member of the serine/threonine kinase family that plays an important role in regulating the mitotic and meiotic cell cycle. The phosphorylation of specific substrates mediated by PLK1 controls nuclear envelope breakdown (NEBD), centrosome maturation, proper spindle assembly, chromosome segregation, and cytokinesis.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein

HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer

Synonyms

PLK-1; STPK13; PLK

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide

Molecular Weight

The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.0ng/ml determined by ELISA. Immobilized Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer is greater than 95% as determined by SEC-HPLC.

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