Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447B)
Catalog: MHC-HM447B
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| Product Description | Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide. |
Product Background |
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer |
| MHC Form | Monomer |
| Allele | HLA-A*02:01 |
Gene/Protein ID |
P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide |
| Molecular Weight | The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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