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Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein, Ultra Low Endotoxin (MHC-HM443B-UL) Summary
Product Description |
Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide. |
Product Background |
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Specifications
Protein |
HLA-A*02:01&B2M&PRAME (SLLQHLIGL) |
Synonyms |
PRAME; OIP-4; OIP4; MAPE; |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLQHLIGL |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and SLLQHLIGL peptide |
Molecular Weight |
The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 51-58 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). |
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Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer (Cat. No.MHC-HM443B) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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