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Biotinylated Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein (MHC-HE041B) Summary
Product Description |
Recombinant Biotinylated Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and TLIRWAILEA peptide. |
Product Background |
TLIRWAILEA is position 344-353 of Foxp3 which is a member of a large forkhead family of related proteins. The peptide has minimal homology with other Foxp family members, such as Foxp1, 2 and 4, and it has also been shown to induce strong peptide-specific CD8+ T cell responses, which recognize Foxp3+/HLA-A*02:01+ cutaneous T lymphoma cells. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Specifications
Protein |
HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) |
Synonyms |
TLIRWAILEA |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&TLIRWAILEA |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and TLIRWAILEA peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 19.7ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 19.7ng/ml determined by ELISA. |
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Biotinylated Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&Foxp3 (TLIRWAILEA) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
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