Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein (MHC-HM463B)
Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein (MHC-HM463B)
Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein (MHC-HM463B)
Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein (MHC-HM463B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and ALLAVGATK peptide
Catalog #MHC-HM463B
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Product Description
 Recombinant Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*03:01), Ile21-Met119 (B2M) and ALLAVGATK peptide.
Product Background
 Immunization against the ALLAVGATK peptide may occur in melanoma patients and that the immunogenicity of this peptide is similar to that of the majority of melanoma differentiation antigens defined to date. Therefore, the use of ALLAVGATK in active immunotherapy for HLA-A3 melanoma patients, for whom no other immunogenic peptide has been so far described, remains a distinct possibility, providing that patients treated in vivo with this peptide develop a better in vitro response than already demonstrated for HLA-A2 epitopes generated from this same antigenic protein.
Product Category
 Recombinant Protein
Target
 HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer
MHC Form
 Monomer
Allele
 HLA-A*03:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and ALLAVGATK peptide
Molecular Weight
 The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*03:01&B2M&gp100 (ALLAVGATK) Monomer is greater than 95% as determined by SEC-HPLC.
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