Name: Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein (MHC-HE042B)
Brand: KactusBio
SKU: MHC-HE042B-100UG
Availability: InStock

Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein (MHC-HE042B)

Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA.

Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVGAVGVGK peptide

Catalog #MHC-HE042B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A03:01), Ile21-Met119(B2M) and VVGAVGVGK peptide.
Product Background	Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer
Synonyms	MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form	Monomer
Allele	HLA-A*03:01
Accession	NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVGAVGVGK peptide
Molecular Weight	The protein has a predicted MW of 35.26 kDa (HLA-A*03:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
	Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.