Name: Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein (MHC-HE039B)
Brand: KactusBio
SKU: MHC-HE039B-100UG
Price: 310.0 USD
Availability: InStock

Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein (MHC-HE039B)

Immobilized Biotinylated HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide

Catalog #MHC-HE039B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A11:01), Ile21-Met119(B2M) and KITDFGRAK peptide.
Product Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
MHC Form	Monomer
Allele	HLA-A*11:01
Accession	AAV53343.1(HLA-A*11:01)&P61769(B2M)&KITDFGRAK
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide
Molecular Weight	The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for HLA-A11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for HLA-A11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer is greater than 95% as determined by SEC-HPLC.