Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) from HEK293

Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*11:01&B2M&EGFR TCR with the EC50 of 3.7ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*11:01&B2M&EGFR TCR with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide

Catalog #MHC-HM479B

Price

$341⁰⁰

Size

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Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein (MHC-HM479B) Summary

Product Description

Recombinant Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide.

Product Background

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Specifications

Protein	HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK)
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2;EC2.7.10; EC2.7.10.1
MHC Form	Monomer
Allele	HLA-A*11:01
Accession	AAV53343.1(HLA-A*11:01)&P61769(B2M)&KITDFGRAK
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide
Molecular Weight	The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A11:01&B2M&EGFR TCR with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human HLA-A11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A11:01&B2M&EGFR TCR with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer is greater than 95% as determined by SEC-HPLC.