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Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein (MHC-HE451B) Summary
Product Description |
Recombinant Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGLAK peptide. |
Product Background |
The human telomerase catalytic subunit human telomerase reverse transcriptase (hTERT) is expressed in many human cancer cells derived from multiple tissues, but infrequently in normal cells. Thus, hTERT is an attractive candidate target for tumor immunotherapy. hTERT:540–548 peptide (p540, ILAKFLHWL) has been identified as an effective HLA-A*0201-restricted T-cell epitope, an effective T-cell-based cancer treatment, including vaccines, against hTERT will likely require the identification of other MHC class I-restricted epitopes in this antigen. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&TERT (ILAKFLHWL) Specifications
Protein |
HLA-A*02:01&B2M&TERT (ILAKFLHWL) |
Synonyms |
TERT; ILAKFLHWL |
Accession |
AAV53343.1(HLA-A*11:01)&P61769(B2M)&KITDFGLAK |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGLAK peptide |
Molecular Weight |
The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 32.6ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HLA-A*11:01&B2M&EGFR TCR, hFc Tag with the EC50 of 32.6ng/ml determined by ELISA. |
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Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR WT (KITDFGLAK) Monomer is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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