• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and IVCPICSQK peptide
Catalog #MHC-HM464B
Price
Regular price $34100
Regular price Sale price $34100
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer Protein (MHC-HM464B) Summary

Product Description

 Recombinant Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and IVCPICSQK peptide.

Product Background

 HPV16 E7 protein, one of the primary target proteins in molecular targeted therapy for HPV-induced cervical cancer. The affitoxin, ZHPV16E7 affitoxin384 was generated by fusing the modified Pseudomonas Exotoxin A (PE38KDEL) to the HPV16 E7-specific affibody.

HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Specifications

Protein
 HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK)
Synonyms
 HPV16; E7; MHC;
MHC Form
 Monomer
Allele
 HLA-A*11:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and IVCPICSQK peptide
Molecular Weight
 The protein has a predicted MW of 50.39 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
Other Available Proteins:
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