Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer Protein (M

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly25-Val309(HLA-B*27:05), Ile21-Met119(B2M) and LRVMMLAPF peptide

Catalog #MHC-HE472B

Price

$341⁰⁰

Size

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Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer Protein (MHC-HE472B) Summary

Product Description

Recombinant Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Val309(HLA-B*27:05), Ile21-Met119(B2M) and LRVMMLAPF peptide.

Product Background

YeiH is a conserved bacterial inner membrane protein widely distributed in gut microflora like Escherichia coli. Its immunogenic segment LRVMMLAPF (YeiH232-240) is an HLA-B*27-bound microbial epitope. By mimicking self-antigens, this bacterial peptide serves as an environmental trigger that activates cross-reactive, pathogenic CD8+ T cells, driving the immune response in conditions such as ankylosing spondylitis (AS) and acute anterior uveitis (AAU).

HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Specifications

Protein	HLA-B*27:05&B2M&YEIH (LRVMMLAPF)
Synonyms	yeiH; b2158; JW2145;
MHC Form	Monomer
Allele	HLA-B*27:05
Accession	AAA36221.1(HLA-B*27:05)&P61769(B2M)&LRVMMLAPF
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Val309(HLA-B*27:05), Ile21-Met119(B2M) and LRVMMLAPF peptide
Molecular Weight	The protein has a predicted MW of 36.08 kDa (HLA-B*27:05) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-B*27:05&B2M&YEIH (LRVMMLAPF) Monomer is greater than 95% as determined by SEC-HPLC.