Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein (MHC-HM466B)
Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein (MHC-HM466B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and GGDPHLPTL peptide
Catalog #MHC-HM466B
Regular price $310.00
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Product Description
Recombinant Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119 (B2M) and GGDPHLPTL peptide.
Product Background
Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is expressed in germinal-center-derived, mononuclear Hodgkin (H) and multinuclear, diagnostic Reed-Sternberg (RS) cells in classical EBV-positive Hodgkin's lymphoma (cHL). LMP1 expression in EBV-negative H-cell lines results in a significantly increased number of RS cells.
Product Category
Recombinant Protein
Target
HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer
MHC Form
Monomer
Allele
HLA-E*01:03
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and GGDPHLPTL peptide
Molecular Weight
The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer is greater than 95% as determined by SEC-HPLC.

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