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Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein (MHC-HM466B) Summary
Product Description |
Recombinant Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119 (B2M) and GGDPHLPTL peptide. |
Product Background |
Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is expressed in germinal-center-derived, mononuclear Hodgkin (H) and multinuclear, diagnostic Reed-Sternberg (RS) cells in classical EBV-positive Hodgkin's lymphoma (cHL). LMP1 expression in EBV-negative H-cell lines results in a significantly increased number of RS cells. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Specifications
Protein |
HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) |
Synonyms |
EBV LMP1; GGDPHLPTL |
MHC Form |
Monomer |
Allele |
HLA-E*01:03 |
Accession |
P13747(HLA-E*01:03)&P61769(B2M)&GGDPHLPTL |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and GGDPHLPTL peptide |
Molecular Weight |
The protein has a predicted MW of 50.15 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-E*01:03&B2M&EBV LMP1 (GGDPHLPTL) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
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