Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein (MHC-HM406B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide
Catalog #MHC-HM406B
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Product Description
 Recombinant Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.
Product Background
 HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Product Category
 Recombinant Protein
Target
 HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer
Synonyms
 HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
MHC Form
 Monomer
Allele
 HLA-E*01:03
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide
Molecular Weight
 The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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