Biotinylated Human HLA-E*01:03 Complex Protein (MHC-HM406B)
Catalog: MHC-HM406B
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| Product Description | Recombinant Biotinylated Human HLA-E*01:03 Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Gly25-Ile305 (HLA-E*01:03), Ile21-Met119 (B2M) and VMAPRTLVL peptide. |
| Product Category | Recombinant Protein |
| Target | HLA-E*01:03 Complex |
| Synonyms | HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
| Gene/Protein ID | P13747 (HLA-E*01:03)&P61769 (B2M)&VMAPRTLVL |
| Species | Human |
| Exact Sequence | Gly25-Ile305 (HLA-E*01:03), Ile21-Met119 (B2M) and VMAPRTLVL peptide |
| Molecular Weight | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human HLA-E*01:03 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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