Name: Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein (MHC-HM406TB)
Brand: KactusBio
SKU: MHC-HM406TB-100UG
Price: 1856.8 USD
Availability: InStock

Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein (MHC-HM406TB)

Immobilized Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.

The purity of Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M)and VMAPRTLVL peptide

Catalog #MHC-HM406TB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-E01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly22-Thr302(HLA-E01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.
Product Background	HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer
Synonyms	HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
MHC Form	Tetramer
Allele	HLA-E*01:03
Accession	P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL
Species	Human
Biotinylated	yes
Amino Acid Range	Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M)and VMAPRTLVL peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.
	The purity of Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC.