Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein (MHC-HM406TB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Exact Sequence: Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M)and VMAPRTLVL peptide

Catalog: MHC-HM406TB

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Product Description Recombinant Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.
Product Category Recombinant Protein
Target HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer
Synonyms HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
Gene/Protein ID P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL
Species Human
Exact Sequence Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M)and VMAPRTLVL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.
The purity of Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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