Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B)
Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B)
Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B)
Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Met1-Glu215
Catalog #HMG-HM1B1B
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Product Description
 Recombinant Biotinylated Human HMGB1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215.
Product Background
 High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.
Product Category
 Recombinant Protein
Target
 HMGB1
Synonyms
 HMGB1; HMG1; HMG3; SBP-1; HMG-1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Glu215
Molecular Weight
 The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC.

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