Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B)

Human AGER, His Tag immobilized on CM5 Chip can bind Biotinylated Human HMGB1, His Tag with an affinity constant of 2.60 uM as determined in SPR assay (Biacore T200).

Biotinylated Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 2.60 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met1-Glu215

Catalog #HMG-HM1B1B

Price

$577⁵⁰

Size

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Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B) Summary

Product Description

Recombinant Biotinylated Human HMGB1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215.

Product Background

High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.

Biotinylated Human HMGB1 Protein (Primary Amine Labeling) (HMG-HM1B1B) Specifications/Details

Protein	HMGB1
Synonyms	HMGB1; HMG1; HMG3; SBP-1; HMG-1;SBP1
Accession	P09429-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Glu215
Molecular Weight	The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 2.60 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human AGER, His Tag immobilized on CM5 Chip can bind Biotinylated Human HMGB1, His Tag with an affinity constant of 2.60 uM as determined in SPR assay (Biacore T200).
	Biotinylated Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC.