Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein (MHC-HM424B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YMLDLQPET peptide

Catalog: MHC-HM424B

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Product Description Recombinant Biotinylated Human HPV16 E7(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YMLDLQPET peptide.

Product Background

 HPV16 E7 protein, one of the primary target proteins in molecular targeted therapy for HPV-induced cervical cancer. The affitoxin, ZHPV16E7 affitoxin384 was generated by fusing the modified Pseudomonas Exotoxin A (PE38KDEL) to the HPV16 E7-specific affibody.

Product Category

 Recombinant Protein

Target

 HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer
Synonyms HPV16; E7; MHC;
MHC Form Monomer
Allele HLA-A*02:01

Gene/Protein ID

 P04439-1(HLA-A*02:01) &P61769(B2M) &YMLDLQPET peptide

Species

 Human

Exact Sequence

 Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YMLDLQPET peptide
Molecular Weight The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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