• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ile27-Lys243
Catalog #IFN-HM140RB
Price
Regular price $57750
Regular price Sale price $57750
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human IFN alpha/beta R2 Protein (IFN-HM140RB) Summary

Product Description

 Recombinant Biotinylated Human IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile27-Lys243.

Product Background

 Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.

Biotinylated Human IFN alpha/beta R2 Protein (IFN-HM140RB) Specifications/Details

Protein
 IFN alpha/beta R2
Synonyms
 IFN-R-2; IFNAR2; IFNARB; IFNABR; IFN-alpha; IFN-alpha/beta R2; IFNbR2; IFN-R; β receptor 2; β receptor10;;IFN-R2;b receptor 2; beta receptor 2; b receptor10; beta receptor10
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile27-Lys243
Molecular Weight
 The protein has a predicted MW of 27.66 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human IFN alpha/beta R2,His-Avi Tag captured on CM5 Chip via Streptavidin can bind Human IFN alpha 1, hFc Tag with an affinity constant of 12.48 nM as determined in SPR assay (Biacore T200). Biotinylated Human IFN alpha/beta R2,His-Avi Tag captured on CM5 Chip via Streptavidin can bind Human IFN alpha 1, hFc Tag with an affinity constant of 12.48 nM as determined in SPR assay (Biacore T200).
Biotinylated Human IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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