Biotinylated Human IFN gamma/IFNG Protein (IFN-HM40GB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln24-Gly161

Catalog: IFN-HM40GB

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Product Description Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161.

Product Background

Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Product Category

Recombinant Protein

Target

IFN gamma/IFNG
Synonyms Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma
Accession

Species

Human

Exact Sequence

Gln24-Gly161
Molecular Weight The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
The purity of Biotinylated Human IFN gamma is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IFN gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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