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Biotinylated Human IFN gamma/IFNG Protein (IFN-HM40GB) Summary
Product Description |
Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161. |
Product Background |
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human IFN gamma/IFNG Protein (IFN-HM40GB) Specifications/Details
Protein |
IFN gamma/IFNG |
Synonyms |
Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ; IFN γ |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln24-Gly161 |
Molecular Weight |
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IFN gamma R1, hFc Tag (Cat. IFN-HM2GR) with the EC50 of 3.7ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human IFN gamma, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IFN gamma R1, hFc Tag (Cat. IFN-HM2GR) with the EC50 of 3.7ng/ml determined by ELISA. |
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Biotinylated Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IFN gamma is greater than 85% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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