Name: Biotinylated Human IgE Protein (IGE-HM401B)
Brand: KactusBio
SKU: IGE-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human IgE Protein (IGE-HM401B)

Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IgE, His Avi Tag with the EC50 of 10.8ng/ml determined by ELISA.

Immobilized Biotinylated Human IgE, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Omalizumab, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.

Biotinylated Human IgE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IgE is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IgE, His Avi Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Cys209-Lys428

Catalog #IGE-HM401B

Size

100UG

500UG

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Biotinylated Human IgE Protein (IGE-HM401B) Summary

Product Description	Recombinant Biotinylated Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Cys209-Lys428.
Product Background	Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human IgE Protein (IGE-HM401B) Specifications/Details

Protein	IgE
Synonyms	Ig epsilon chain C region;IgE
Accession	P01854-1
Species	Human
Biotinylated	yes
Amino Acid Range	Cys209-Lys428
Molecular Weight	The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IgE, His Avi Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IgE, His Avi Tag with the EC50 of 10.8ng/ml determined by ELISA.
	Immobilized Biotinylated Human IgE, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Omalizumab, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
	Biotinylated Human IgE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IgE is greater than 95% as determined by SEC-HPLC.