Biotinylated Human IgE Protein (IGE-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IgE, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Cys209-Lys428

Catalog: IGE-HM401B

$525.00
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Product Description Recombinant Biotinylated Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Cys209-Lys428.

Product Background

Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Product Category

Recombinant Protein

Target

IgE

Gene/Protein ID

P01854-1

Species

Human

Exact Sequence

Cys209-Lys428
Molecular Weight The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human IgE, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human IgE, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
The purity of Biotinylated Human IgE is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IgE on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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