Name: Biotinylated Human IL-1 alpha/IL-1A Protein (IL1-HM40AB)
Brand: KactusBio
SKU: IL1-HM40AB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-1 alpha/IL-1A Protein (IL1-HM40AB)

Human IL-1R1, No Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His-Avi Tag with an affinity constant of 1.01 uM as determined in SPR assay (Biacore T200).

Biotinylated Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.01 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser113-Ala271

Catalog #IL1-HM40AB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-1 alpha/IL-1A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser113-Ala271.
Product Background	The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-1 alpha/IL-1A
Synonyms	Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1
Accession	P01583
Species	Human
Biotinylated	yes
Amino Acid Range	Ser113-Ala271
Molecular Weight	The protein has a predicted MW of 20.95 kDa. Due to glycosylation, the protein migrates to 25-32 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 1.01 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-1R1, No Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His-Avi Tag with an affinity constant of 1.01 uM as determined in SPR assay (Biacore T200).
	Biotinylated Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.