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Biotinylated Human IL-1 alpha/IL-1A Protein (IL1-HM40AB) Summary
Product Description |
Recombinant Biotinylated Human IL-1 alpha/IL-1A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser113-Ala271. |
Product Background |
The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human IL-1 alpha/IL-1A Protein (IL1-HM40AB) Specifications/Details
Protein |
IL-1 alpha/IL-1A |
Synonyms |
Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser113-Ala271 |
Molecular Weight |
The protein has a predicted MW of 20.95 kDa. Due to glycosylation, the protein migrates to 25-32 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.01 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human IL-1R1, No Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His-Avi Tag with an affinity constant of 1.01 uM as determined in SPR assay (Biacore T200). |
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Biotinylated Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-1 alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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