Biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40BB)
Catalog: IL1-HE40BB
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Product Description | Recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus.It contains Ala117-Ser269. |
Product Background |
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
Product Category |
Recombinant Protein |
Target |
IL-1 Beta/IL-1F2 |
Synonyms | Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B |
Gene/Protein ID |
P01584 |
Species |
Human |
Exact Sequence |
Ala117-Ser269 |
Molecular Weight | The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-1R2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 13.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
E.coli |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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