Biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40BB)

  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-1 RII, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala117-Ser269

Catalog: IL1-HE40BB

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Product Description Recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-terminal.; It contains Ala117-Ser269.
Product Category Recombinant Protein
Target IL-1 Beta/IL-1F2
Synonyms Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B
Gene/Protein ID P01584
Species Human
Exact Sequence Ala117-Ser269
Molecular Weight The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-1 RII, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-1 RII, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7.6ng/ml determined by ELISA.
The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-1 Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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