Biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40BB)

  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-1R2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 13.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala117-Ser269

Catalog: IL1-HE40BB

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Product Description Recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus.It contains Ala117-Ser269.

Product Background

 Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.

Product Category

 Recombinant Protein

Target

 IL-1 Beta/IL-1F2
Synonyms Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B

Gene/Protein ID

 P01584

Species

 Human

Exact Sequence

 Ala117-Ser269
Molecular Weight The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Human IL-1R2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 13.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-1R2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 13.1ng/ml determined by ELISA.
The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-1 Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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