Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB)
Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: His22-Asn235
Catalog #IL1-HM4RAB
Regular price $525.00
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Product Description
Recombinant Biotinylated Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His22-Asn235.
Product Background
The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.
Product Category
Recombinant Protein
Target
IL-10R alpha
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
His22-Asn235
Molecular Weight
The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA.
Biotinylated Human IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

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