Biotinylated Human IL-12 Protein (IL1-HM412B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)

Catalog: IL1-HM412B

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Product Description	Recombinant Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) and Arg23-Ser219 (IL-12A).
Product Background	Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Product Category	Recombinant Protein

Target	IL-12
Synonyms	CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
Gene/Protein ID	P29460(IL-12B)&P29459(IL-12A)
Species	Human
Exact Sequence	Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Molecular Weight	The protein has a predicted MW of 34.7 kDa (IL-12B) and 25.5 kDa (IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 10.8ng/ml determined by ELISA.
	The purity of Bitinylated Human IL-12 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.