Biotinylated Human IL-12 Protein (IL1-HM412B)
Biotinylated Human IL-12 Protein (IL1-HM412B)
Biotinylated Human IL-12 Protein (IL1-HM412B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Catalog #IL1-HM412B
Regular price $750.00
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Product Description
Recombinant Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) and Arg23-Ser219 (IL-12A).
Product Background
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Product Category
Recombinant Protein
Target
IL-12
Synonyms
CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Molecular Weight
The protein has a predicted MW of 34.7 kDa (IL-12B) and 25.5 kDa (IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 10.8ng/ml determined by ELISA.
Biotinylated Human IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Bitinylated Human IL-12 is greater than 95% as determined by SEC-HPLC.

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