Biotinylated Human IL-13 (E31K) Protein (IL1-HM4M1B)

Immobilized Biotinylated Human IL-13 (E31K), His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 76.6ng/ml determined by ELISA.

Biotinylated Human IL-13 (E31K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-13 (E31K) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-13 (E31K), His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 76.6ng/ml determined by ELISA.
Amino Acid Range: Gly21-Asn132(E31K)

Catalog #IL1-HM4M1B

Price

$578⁰⁰

Size

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Biotinylated Human IL-13 (E31K) Protein (IL1-HM4M1B) Summary

Product Description

Recombinant Biotinylated Human IL-13 (E31K) Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Gly21-Asn132 (E31K).

Product Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Biotinylated Human IL-13 (E31K) Protein (IL1-HM4M1B) Specifications/Details

Protein	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789;Interleukin-13; IL 13
Accession	AAK53823
Species	Human
Biotinylated	no
Amino Acid Range	Gly21-Asn132(E31K)
Molecular Weight	The protein has a predicted MW of 15.25 kDa. Due to glycosylation, the protein migrates to 32-52 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-13 (E31K), His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 76.6ng/ml determined by ELISA.
Product Tag	N-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human IL-13 (E31K), His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 76.6ng/ml determined by ELISA.
	Biotinylated Human IL-13 (E31K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-13 (E31K) is greater than 95% as determined by SEC-HPLC.