Product Description |
Recombinant Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile31-Thr205. |
Product Background |
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
Product Category |
Recombinant Protein |
Protein |
IL-15RA/IL-15 R alpha/CD215 |
Synonyms |
IL-15 R alpha; CD215; IL-15RA; MGC104179 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile31-Thr205 |
Molecular Weight |
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-15 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA, His Tag with the EC50 of 55.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-15 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA, His Tag with the EC50 of 55.8ng/ml determined by ELISA. |
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Biotinylated Human IL-15RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-15RA is greater than 95% as determined by SEC-HPLC. |
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