• Express System: HEK293
  • Product Tag: N-HA, N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-17A&F, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 16.9ng/ml determined by ELISA.
  • Amino Acid Range: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Catalog #ILF-HM420B
Price
Regular price $578.00
Regular price Sale price $578.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-17A&F Protein (ILF-HM420B) Summary

Product Description

 Recombinant Biotinylated Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A is fused with a HA tag at the N-terminus and subunit IL-17F is fused with a His tag and a Avi tag at the N-terminus.It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F).

Product Background

 Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human IL-17A&F Protein (ILF-HM420B) Specifications/Details

Protein
 IL-17A&F
Synonyms
 IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Molecular Weight
 The protein has a predicted MW of 16.35 kDa (IL-17A) and 17.81 kDa (IL-17F). Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human IL-17A&F, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 16.9ng/ml determined by ELISA.
Product Tag
 N-HA, N-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human IL-17A&F, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 16.9ng/ml determined by ELISA. Immobilized Biotinylated Human IL-17A&F, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 16.9ng/ml determined by ELISA.
Immobilized Biotinylated Human IL-17A&F, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. Immobilized Biotinylated Human IL-17A&F, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Biotinylated Human IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17A&F is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-17A&F is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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