Biotinylated Human IL-17R alpha/CD217 Protein (IL1-HM5RAB)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-17A&F, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.70_g/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu33-Trp320

Catalog: IL1-HM5RAB

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Product Description Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Leu33-Trp320.
Product Category Recombinant Protein
Target IL-17R alpha/CD217
Synonyms CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-7R; IL-7R-alpha; ILRA
Gene/Protein ID Q96F46-1
Species Human
Exact Sequence Leu33-Trp320
Molecular Weight The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-17A&F, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.70_g/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-17A&F, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.70_g/ml determined by ELISA.
Immobilized Human IL-17F, His Tag at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76_g/ml determined by ELISA.
The purity of Biotinylated Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-17R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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