Biotinylated Human IL-17R alpha/CD217 Protein (IL1-HM5RAB)
Biotinylated Human IL-17R alpha/CD217 Protein (IL1-HM5RAB)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu33-Trp320
Catalog #IL1-HM5RAB
Regular price $525.00
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Product Description

Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Leu33-Trp320.

Product Background

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-17R alpha/CD217

Synonyms

CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Leu33-Trp320

Molecular Weight

The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA. Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA.
Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA. Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA.
Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17R alpha is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.

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