• Express System: HEK293
  • Product Tag: C-hFc (IgG1)-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-17A, His Tag (Cat. ILA-HM117) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.24ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Leu33-Trp320
Catalog #IL1-HM5RAB
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-17R alpha/CD217 Protein (IL1-HM5RAB) Summary

Product Description

 Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu33-Trp320.

Product Background

 Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.

Biotinylated Human IL-17R alpha/CD217 Protein (IL1-HM5RAB) Specifications/Details

Protein
 IL-17R alpha/CD217
Synonyms
 CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα;IL17RA; IL17R-alpha;IL17R alpha
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu33-Trp320
Molecular Weight
 The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17A, His Tag (Cat. ILA-HM117) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.24ug/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-17A, His Tag (Cat. ILA-HM117) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.24ug/ml determined by ELISA (QC Test). Immobilized Human IL-17A, His Tag (Cat. ILA-HM117) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc-Avi Tag with the EC50 of 0.24ug/ml determined by ELISA (QC Test).
Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA. Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 0.94ug/ml determined by ELISA.
Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA. Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA.
Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Biotinylated Human IL-17R alpha, hFc-Avi Tag on Steptavidin-Biosensor can bind Human IL-17A, His Tag with an affinity constant of 5.73 nM as determined in BLI assay. Loaded Biotinylated Human IL-17R alpha, hFc-Avi Tag on Steptavidin-Biosensor can bind Human IL-17A, His Tag with an affinity constant of 5.73 nM as determined in BLI assay.
The purity of Biotinylated Human IL-17R alpha is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human IL-17R alpha is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...