Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr37-Asp193

Catalog: IL1-HE018B

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Product Description Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Tyr37-Asp193.

Product Background

 Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Product Category

 Recombinant Protein

Target

 IL-18
Synonyms IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1

Gene/Protein ID

 Q14116

Species

 Human

Exact Sequence

 Tyr37-Asp193
Molecular Weight The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

 No Tag

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2ng/ml determined by ELISA.
The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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