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Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B) Summary
Product Description |
Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.; It contains Tyr37-Asp193. |
Product Background |
Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B) Specifications/Details
Protein |
IL-18 |
Synonyms |
IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Tyr37-Asp193 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. The affinity constant of 3.46 nM as determined in SPR assay (Biacore T200). |
Product Tag |
No Tag |
Fluorophore |
Biotin-labeled |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human IL-18 R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Biotinylated Human IL-18, No Tag with an affinity constant of 3.46 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. |
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Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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