Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr37-Asp193

Catalog: IL1-HE018B

$525.00
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Product Description Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.; It contains Tyr37-Asp193.

Product Background

 Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Product Category

 Recombinant Protein

Target

 IL-18
Synonyms IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1
Accession

Species

 Human

Amino Acid Range

 Tyr37-Asp193
Molecular Weight The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. See testing image for detail.

Product Tag

 No Tag

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA.
The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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