Name: Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B)
Brand: KactusBio
SKU: IL1-HE018B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B)

Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA.

Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
Activity: Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Tyr37-Asp193

Catalog #IL1-HE018B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.; It contains Tyr37-Asp193.
Product Background	Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-18
Synonyms	IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ
Accession	Q14116
Species	Human
Biotinylated	yes
Amino Acid Range	Tyr37-Asp193
Molecular Weight	The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA.
	Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.