Product Description |
Recombinant Biotinylated Human IL-18 R1/CD218a Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Arg329. |
Product Background |
Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
Product Category |
Recombinant Protein |
Target |
IL-18 R1/CD218a |
Synonyms |
CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala19-Arg329 |
Molecular Weight |
The protein has a predicted MW of 38.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.24 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 1.24 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Human IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-18 R1 is greater than 95% as determined by SEC-HPLC. |
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