Biotinylated Human IL-18 R1/CD218a Protein (IL8-HM4R1B)
Catalog: IL8-HM4R1B
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| Product Description | Recombinant Biotinylated Human IL-18 R1/CD218a Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala19-Arg329. |
| Product Category | Recombinant Protein |
| Target | IL-18 R1/CD218a |
| Synonyms | CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra |
| Gene/Protein ID | AAH69575 |
| Species | Human |
| Exact Sequence | Ala19-Arg329 |
| Molecular Weight | The protein has a predicted MW of 38.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 1.4nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human IL-18 R1, His Tag captured on CM5-SA Chip can bind Human IL-18, No Tag with an affinity constant of 1.4nM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human IL-18 R1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human IL-18 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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