Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)
Catalog: IL1-HM4R3B
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| Product Description | Recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359. |
Product Background |
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. |
Product Category |
Recombinant Protein |
Target |
IL-1R3/IL-1 RAcP |
| Synonyms | IL1RAP; C3orf13; IL-1 R3; IL-1 RAcP |
| Accession | |
Species |
Human |
Amino Acid Range |
Ser21-Glu359 |
| Molecular Weight | The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. |
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The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human IL-1R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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