Name: Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)
Brand: KactusBio
SKU: IL1-HM4R3B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)

Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.

Biotinylated Human IL-1R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser21-Glu359

Catalog #IL1-HM4R3B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359.
Product Background	IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-1R3/IL-1 RAcP
Synonyms	IL1RAP; IL-1RAP; C3orf13; IL-1 R3; IL-1 RAcP
Accession	Q9NPH3-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ser21-Glu359
Molecular Weight	The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
	Biotinylated Human IL-1R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC.