Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)
Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)
Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser21-Glu359
Catalog #IL1-HM4R3B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359.
Product Background
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
Product Category
Recombinant Protein
Target
IL-1R3/IL-1 RAcP
Synonyms
IL1RAP; IL-1RAP; C3orf13; IL-1 R3; IL-1 RAcP
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ser21-Glu359
Molecular Weight
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Biotinylated Human IL-1R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC.

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