Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IL-1R3 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser21-Glu359

Catalog: IL1-HM4R3B

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Product Description Recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359.

Product Background

IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.

Product Category

Recombinant Protein

Target

IL-1R3/IL-1 RAcP
Synonyms IL1RAP; C3orf13; IL-1 R3; IL-1 RAcP

Gene/Protein ID

Q9NPH3-1

Species

Human

Exact Sequence

Ser21-Glu359
Molecular Weight The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human IL-1R3 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IL-1R3 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-1R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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