Biotinylated Human IL-2 Protein (IL2-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 59.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala21-Thr153

Catalog: IL2-HM401B

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Product Description Recombinant Biotinylated Human IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala21-Thr153.

Product Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

Product Category

Recombinant Protein

Target

IL-2
Accession

Species

Human

Amino Acid Range

Ala21-Thr153
Molecular Weight The protein has a predicted MW of 18.32 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 59.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 59.6ng/ml determined by ELISA.
The purity of Biotinylated Human IL-2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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