Biotinylated Human IL-2 Protein (IL2-HM401B)
Biotinylated Human IL-2 Protein (IL2-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2 R alpha, His Tag (Cat. CD5-HM125) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 93.3ng/ml determined by ELISA.
  • Amino Acid Range: Ala21-Thr153
Catalog #IL2-HM401B
Regular price $577.50
/
Size

Order by 4pm ET for delivery on:

Biotinylated Human IL-2 Protein (IL2-HM401B) Summary

Product Description

Recombinant Biotinylated Human IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala21-Thr153.

Product Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human IL-2 Protein (IL2-HM401B) Specifications/Details

Protein
IL-2
Synonyms
IL2; T-cell growth factor; TCGF; Aldesleukin
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala21-Thr153
Molecular Weight
The protein has a predicted MW of 18.32 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-2 R alpha, His Tag (Cat. CD5-HM125) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 93.3ng/ml determined by ELISA.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-2 R alpha, His Tag (Cat. CD5-HM125) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 93.3ng/ml determined by ELISA. Immobilized Human IL-2 R alpha, His Tag (Cat. CD5-HM125) at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 93.3ng/ml determined by ELISA.
Biotinylated Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-2 is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Recommended Products

Recently viewed