Biotinylated Human IL-2 Protein (IL2-HM401B)
Biotinylated Human IL-2 Protein (IL2-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala21-Thr153
Catalog #IL2-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala21-Thr153.
Product Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Product Category
Recombinant Protein
Target
IL-2
Synonyms
IL2; T-cell growth factor; TCGF; Aldesleukin
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala21-Thr153
Molecular Weight
The protein has a predicted MW of 18.32 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
Biotinylated Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-2 is greater than 95% as determined by SEC-HPLC.

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