Product Description |
Recombinant Biotinylated Human IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala21-Thr153. |
Product Background |
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. |
Product Category |
Recombinant Protein |
Target |
IL-2 |
Synonyms |
IL2; T-cell growth factor; TCGF; Aldesleukin |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala21-Thr153 |
Molecular Weight |
The protein has a predicted MW of 18.32 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). |
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Biotinylated Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-2 is greater than 95% as determined by SEC-HPLC. |
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