Name: Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)
Brand: KactusBio
SKU: CD1-HM422B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)

Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 uM as determined in SPR assay (Biacore T200).

Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-15Ra&IL-15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 38.0ng/ml determined by ELISA (QC Test).

Biotinylated Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-15Ra&IL-15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 38.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.079 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala27-Asp239

Catalog #CD1-HM422B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Asp239.
Product Background	IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-2 R beta/CD122
Synonyms	CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6; IL-15 R β; IL-2 R β; IL-2R subunit β
Accession	NP_000869
Species	Human
Biotinylated	yes
Amino Acid Range	Ala27-Asp239
Molecular Weight	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-15Ra&IL-15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 38.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.079 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 uM as determined in SPR assay (Biacore T200).
	Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-15Ra&IL-15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 38.0ng/ml determined by ELISA (QC Test).
	Biotinylated Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.