Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 0.079 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala27-Asp239

Catalog: CD1-HM422B

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Product Description Recombinant Biotinylated Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Asp239.
Product Category Recombinant Protein
Target IL-2 R beta/CD122
Synonyms CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6
Gene/Protein ID NP_000869
Species Human
Exact Sequence Ala27-Asp239
Molecular Weight The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.079 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 uM as determined in SPR assay (Biacore T200) (QC Test).
Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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