Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 0.101 _M as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala27-Asp239

Catalog: CD1-HM422B

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Product Description Recombinant Biotinylated Human IL-2 R beta/CD122 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala27-Asp239.
Product Category Recombinant Protein
Target IL-2 R beta/CD122
Synonyms CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6
Gene/Protein ID NP_000869
Species Human
Exact Sequence Ala27-Asp239
Molecular Weight The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.101 _M as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.101 _M as determined in SPR assay (Biacore T200).
Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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