Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)
Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)
Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)
Biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15Ra&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). The affinity constant of 0.079 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala27-Asp239
Catalog #CD1-HM422B
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Product Description
 Recombinant Biotinylated Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Asp239.
Product Background
 IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
Product Category
 Recombinant Protein
Target
 IL-2 R beta/CD122
Synonyms
 CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala27-Asp239
Molecular Weight
 The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-15Ra&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). The affinity constant of 0.079 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 uM as determined in SPR assay (Biacore T200).
Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15Ra&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Biotinylated Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
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