Product Description |
Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus.It contains His16-Thr228 (IL-22R alpha 1) and Met20-Ser220 (IL-10R beta). |
Product Background |
IL-22Rα1&IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL-22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL10 Rβ or IL20 Rβ and the respective ligands is required for signal transduction[1]. |
Product Category |
Recombinant Protein |
Target |
IL-22R alpha 1&IL-10R beta |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta) |
Molecular Weight |
The protein has a predicted MW of 52.07 kDa (IL-22R alpha 1) and 48.85 kDa (IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 1.36ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 1.36ug/ml determined by ELISA. |
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Biotinylated Human IL-22R alpha 1&IL-10R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-22R alpha 1&IL-10R beta is greater than 95% as determined by SEC-HPLC. |
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