Biotinylated Human IL-22R alpha 1&IL-10R beta Protein (ILR-HM5ABB)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-22, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta)

Catalog: ILR-HM5ABB

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Product Description Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from Expi293 with hFc tag and Avi Tag at the C-terminal.It contains His16-Thr228(IL-22R alpha 1)&Met20-Ser220(IL-10R beta).
Product Category Recombinant Protein
Target IL-22R alpha 1&IL-10R beta
Gene/Protein ID Q8N6P7(IL-22R alpha 1)&Q08334(IL-10R beta)
Species Human
Exact Sequence His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta)
Molecular Weight The protein has a predicted MW of 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-22, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-22, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA.
The purity of Biotinylated Human IL-22R alpha 1&IL-10R beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-22R alpha 1&IL-10R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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