Biotinylated Human IL-22R alpha 1&IL-10R beta Protein (ILR-HM5ABB)
Catalog: ILR-HM5ABB
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Product Description | Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from Expi293 with hFc tag and Avi Tag at the C-terminal.It contains His16-Thr228(IL-22R alpha 1)&Met20-Ser220(IL-10R beta). |
Product Category | Recombinant Protein |
Target | IL-22R alpha 1&IL-10R beta |
Gene/Protein ID | Q8N6P7(IL-22R alpha 1)&Q08334(IL-10R beta) |
Species | Human |
Exact Sequence | His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta) |
Molecular Weight | The protein has a predicted MW of 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human IL-22, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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