Biotinylated Human IL-23 alpha&IL-12 beta Protein (IL2-HM4ABB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Anti-IL-23 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)

Catalog: IL2-HM4ABB

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Product Description Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Arg20-Pro189(IL-23 alpha)&Ile23-Ser328(IL-12 beta).
Product Category Recombinant Protein
Target IL-23 alpha&IL-12 beta
Gene/Protein ID Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)
Species Human
Exact Sequence Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)
Molecular Weight The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-IL-23 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-IL-23 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test).
Immobilized Human IL-23R, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 0.87μg/ml determined by ELISA.
Biotinylated Human IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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