Biotinylated Human IL-23 alpha&IL-12 beta Protein (IL2-HM4ABB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)

Catalog: IL2-HM4ABB

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Product Description Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg20-Pro189(IL-23 alpha)&Ile23-Ser328(IL-12 beta).

Product Background

The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.

Product Category

Recombinant Protein

Target

IL-23 alpha&IL-12 beta

Gene/Protein ID

Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)

Species

Human

Exact Sequence

Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)
Molecular Weight The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA.
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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