Product Description |
Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg20-Pro189 (IL-23 alpha) and Ile23-Ser328 (IL-12 beta). |
Product Background |
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity. |
Product Category |
Recombinant Protein |
Target |
IL-23 alpha&IL-12 beta |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta) |
Molecular Weight |
The protein has a predicted MW of 21.7 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
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Biotinylated Human IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC. |
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