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Biotinylated Human IL-23 alpha&IL-12 beta Protein (IL2-HM4ABB) Summary
Product Description |
Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of IL-23 alpha.It contains Arg20-Pro189 (IL-23 alpha) and Ile23-Ser328 (IL-12 beta). |
Product Background |
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human IL-23 alpha&IL-12 beta Protein (IL2-HM4ABB) Specifications/Details
Protein |
IL-23 alpha&IL-12 beta |
Synonyms |
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β; IL-23A&IL-12B; IL23A&IL12B |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta) |
Molecular Weight |
The protein has a predicted MW of 21.7 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
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Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Risankizumab, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. |
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Biotinylated Human IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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