Name: Biotinylated Human IL-3 Protein (IL3-HE401B)
Brand: KactusBio
SKU: IL3-HE401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-3 Protein (IL3-HE401B)

Immobilized Biotinylated Human IL-3, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA (QC Test).

Biotinylated Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-3, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala20-Phe152

Catalog #IL3-HE401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Ala20-Phe152.
Product Background	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-3
Synonyms	IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Accession	P08700
Species	Human
Biotinylated	yes
Amino Acid Range	Ala20-Phe152
Molecular Weight	The protein has a predicted MW of 16.9 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-3, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human IL-3, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA (QC Test).
	Biotinylated Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC.