Biotinylated Human IL-3 Protein (IL3-HE401B)
Catalog: IL3-HE401B
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| Product Description | Recombinant Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-terminal.It contains Ala20-Phe152. |
| Product Category | Recombinant Protein |
| Target | IL-3 |
| Synonyms | IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF |
| Gene/Protein ID | P08700 |
| Species | Human |
| Exact Sequence | Ala20-Phe152 |
| Molecular Weight | The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human IL-3, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human IL-3, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 74.2ng/ml determined by ELISA. |
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The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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