• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-33, His Avi Tag with the EC50 of 4.8ng/ml determined by ELISA.
  • Amino Acid Range: His109-Thr270
Catalog #IL3-HM433B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-33 Protein (IL3-HM433B) Summary

Product Description

 Recombinant Biotinylated Human IL-33 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains His109-Thr270.

Product Background

 Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.

Biotinylated Human IL-33 Protein (IL3-HM433B) Specifications/Details

Protein
 IL-33
Synonyms
 C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2;C9orf26Z;Interleukin-33; Interleukin 33
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 His109-Thr270
Molecular Weight
 The protein has a predicted MW of 21.24 kDa. Due to glycosylation, the protein migrates to 26-36 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-33, His Avi Tag with the EC50 of 4.8ng/ml determined by ELISA.
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-33, His Avi Tag with the EC50 of 4.8ng/ml determined by ELISA. Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-33, His Avi Tag with the EC50 of 4.8ng/ml determined by ELISA.
Immobilized Biotinylated Human IL-33, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Itepekimab, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. Immobilized Biotinylated Human IL-33, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Itepekimab, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA.
Biotinylated Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-33 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-33 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...