Biotinylated Human IL-4 Protein (IL4-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human IL-4R alpha, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: His25-Ser153

Catalog: IL4-HM401B

$750.00
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Product Description Recombinant Biotinylated Human IL-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains His25-Ser153.
Product Category Recombinant Protein
Target IL-4
Synonyms IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
Gene/Protein ID P05112-1
Species Human
Exact Sequence His25-Ser153
Molecular Weight The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-4R alpha, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-4R alpha, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Biotinylated IL-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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