Product Description |
Recombinant Biotinylated Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134. |
Product Background |
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA). |
Product Category |
Recombinant Protein |
Protein |
IL-5 |
Synonyms |
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile20-Ser134 |
Molecular Weight |
The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 68.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-5, His-Avi Tag with an affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 68.2ng/ml determined by ELISA (QC Test). |
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Biotinylated Human IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-5 is greater than 95% as determined by SEC-HPLC. |
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