Biotinylated Human IL-5 Protein (IL5-HM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 68.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile20-Ser134
Catalog #IL5-HM401B
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Product Description
 Recombinant Biotinylated Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134.
Product Background
 IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Product Category
 Recombinant Protein
Target
 IL-5
Synonyms
 Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile20-Ser134
Molecular Weight
 The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 68.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-5, His-Avi Tag with an affinity constant of 7.57 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-5 R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-5, His Tag with the EC50 of 68.2ng/ml determined by ELISA (QC Test).
Biotinylated Human IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-5 is greater than 95% as determined by SEC-HPLC.

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