Biotinylated Human IL-5 R alpha/CD125 Protein (ILR-HM45RB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp21-Glu335

Catalog: ILR-HM45RB

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Product Description Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Asp21-Glu335.
Product Category Recombinant Protein
Target IL-5 R alpha/CD125
Synonyms CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560
Gene/Protein ID Q01344-1
Species Human
Exact Sequence Asp21-Glu335
Molecular Weight The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-5 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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