• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA.
  • Amino Acid Range: Asp21-Glu335
Catalog #ILR-HM45RB
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-5 R alpha/CD125 Protein (ILR-HM45RB) Summary

Product Description

 Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp21-Glu335.

Product Background

 Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.

Biotinylated Human IL-5 R alpha/CD125 Protein (ILR-HM45RB) Specifications/Details

Protein
 IL-5 R alpha/CD125
Synonyms
 CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560;IL5R alpha; IL5R subunit alpha
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Asp21-Glu335
Molecular Weight
 The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA. Immobilized Human IL-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA.
Biotinylated Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...