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Biotinylated Human IL-5 R alpha/CD125 Protein, Ultra Low Endotoxin (ILR-HM45RB-UL) Summary
Product Description |
Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp21-Glu335. |
Product Background |
Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human IL-5 R alpha/CD125 Protein, Ultra Low Endotoxin (ILR-HM45RB-UL) Specifications/Details
Protein |
IL-5 R alpha/CD125 |
Synonyms |
CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp21-Glu335 |
Molecular Weight |
The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA. |
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Biotinylated Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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