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Biotinylated Human IL-6 Protein (IL6-HM401B) Summary
Product Description |
Recombinant Biotinylated Human IL-6 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Val30-Met212. |
Product Background |
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human IL-6 Protein (IL6-HM401B) Specifications/Details
Protein |
IL-6 |
Synonyms |
IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Val30-Met212 |
Molecular Weight |
The protein has a predicted MW of 23.72 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-6, His-Avi Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test). |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-6, His-Avi Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test). |
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Biotinylated Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-6 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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