• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.78ug/ml determined by ELISA.
  • Amino Acid Range: Leu20-Pro365
Catalog #ILR-HM46RB
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46RB) Summary

Product Description

 Recombinant Biotinylated Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.

Product Background

 The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.

Biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46RB) Specifications/Details

Protein
 IL-6 R alpha/CD126
Synonyms
 IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80;IL6R subunit alpha; IL6R-alpha; IL6RA; IL6R1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu20-Pro365
Molecular Weight
 The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.78ug/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.78ug/ml determined by ELISA. Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.78ug/ml determined by ELISA.
Biotinylated Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...