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Biotinylated Human IL-7 R alpha/CD127 Protein, Ultra Low Endotoxin (IL7-HM4RAB-UL) Summary
Product Description |
Recombinant Biotinylated Human IL-7 R alpha/CD127 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu21-Asp239. |
Product Background |
Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human IL-7 R alpha/CD127 Protein, Ultra Low Endotoxin (IL7-HM4RAB-UL) Specifications/Details
Protein |
IL-7 R alpha/CD127 |
Synonyms |
IL-7R alpha; IL7Ra; CD127; CDW127; IL7Rα |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Glu21-Asp239 |
Molecular Weight |
The protein has a predicted MW of 28.16 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 7.37 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human IL-7R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 7.37 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Human IL-7 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IL-7 R alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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